Group O blood donors in Iran: evaluation of isoagglutinin titers and immunoglobulin G subclasses

Abstract This study aims to determine the most frequent titers of anti-A and anti-B (both presumed immunoglobulin [Ig]M IgG) in Iranian group O blood donors compare these titer values with those found other studies. In addition, alloantibody production plasma levels four IgG subclasses were compared between high-titer non–high-titer groups. investigated 358 samples. Based on results, two groups (high-titer non–high-titer) formed. Antibody detection tests performed detect unexpected antibodies D, C, c, E, e, K, k, Fy a , b Jk M, N, S, s, P1, Le . Four also evaluated through nephelometry assay. The obtained by room temperature indirect antiglobulin tube was 256. frequency ≥512 31.5 percent. None cases showed RBC alloantibodies. IgG2 significantly higher group. Evaluation isoagglutinin can provide insight into both within population as populations. A significant difference identified. More investigation needs be conducted root cause this finding. Immunohematology 2021;37:5–12